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inducible cas9 expression construct pcw cas9  (Addgene inc)


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    Addgene inc inducible cas9 expression construct pcw cas9
    Inducible Cas9 Expression Construct Pcw Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 349 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inducible+cas9+expression+construct+pcw+cas9/pCW-Cas9+(Plasmid+%2350661)/pmc10089214__pnas__2218330120__sapp-27-8-13
    Average 96 stars, based on 349 article reviews
    inducible cas9 expression construct pcw cas9 - by Bioz Stars, 2026-11
    96/100 stars

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    Expressing:

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma
    Article Snippet: .. Expression vectors and sgRNA design The inducible Cas9 expression construct pCW-Cas9 was acquired from Addgene (Addgene: 50661) ( Wang et al., 2014 ). .. The pLKO5-sgRNA-EFS-GFP (Addgene: 57822) and pLKO5-sgRNA-EFS-tRFP (Addgene: 57823) vectors ( Heckl et al., 2014 ) were used to clone sgRNAs targeting early exons and/or active domains of the human CREBBP and EP300 genes, and a neutral control region in the PPP1R12C intron 1, as described ( Sanjana et al., 2014 ; Shalem et al., 2014 ).

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma
    Article Snippet: .. The inducible Cas9 expression construct pCW-Cas9 was acquired from Addgene (Addgene: 50661) ( Wang et al., 2014 ). .. The pLKO5-sgRNA-EFS-GFP (Addgene: 57822) and pLKO5-sgRNA-EFS-tRFP (Addgene: 57823) vectors ( Heckl et al., 2014 ) were used to clone sgRNAs targeting early exons and/or active domains of the human CREBBP and EP300 genes, and a neutral control region in the PPP1R12C intron 1, as described ( Sanjana et al., 2014 ; Shalem et al., 2014 ).

    Article Title: SUPPLEMENTARY INFORMATION KMT2D acetylation by CREBBP reveals a cooperative functional interaction in normal and malignant germinal center B cells
    Article Snippet: .. For the construction of isogenic cell lines, the inducible Cas9 expression construct pCW-Cas9 (Addgene: #50661), pLKO5-sgRNA- EFS-GFP (Addgene: #57822), pLKO5-sgRNA-EFS-tRFP (Addgene: #57823), and pHIVZsgreen (Addgene: #18121) were acquired from Addgene 6,7. .. Benchling (http://benchling.com) was used to design sgRNAs targeting active domains of the human CREBBP, EP300 or KMT2D genes, as well as a neutral control region in the PPP1R12C intron 1 (at least two independent sgRNAs/gene).

    Construct:

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma
    Article Snippet: .. Expression vectors and sgRNA design The inducible Cas9 expression construct pCW-Cas9 was acquired from Addgene (Addgene: 50661) ( Wang et al., 2014 ). .. The pLKO5-sgRNA-EFS-GFP (Addgene: 57822) and pLKO5-sgRNA-EFS-tRFP (Addgene: 57823) vectors ( Heckl et al., 2014 ) were used to clone sgRNAs targeting early exons and/or active domains of the human CREBBP and EP300 genes, and a neutral control region in the PPP1R12C intron 1, as described ( Sanjana et al., 2014 ; Shalem et al., 2014 ).

    Article Title: Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma
    Article Snippet: .. The inducible Cas9 expression construct pCW-Cas9 was acquired from Addgene (Addgene: 50661) ( Wang et al., 2014 ). .. The pLKO5-sgRNA-EFS-GFP (Addgene: 57822) and pLKO5-sgRNA-EFS-tRFP (Addgene: 57823) vectors ( Heckl et al., 2014 ) were used to clone sgRNAs targeting early exons and/or active domains of the human CREBBP and EP300 genes, and a neutral control region in the PPP1R12C intron 1, as described ( Sanjana et al., 2014 ; Shalem et al., 2014 ).

    Article Title: SUPPLEMENTARY INFORMATION KMT2D acetylation by CREBBP reveals a cooperative functional interaction in normal and malignant germinal center B cells
    Article Snippet: .. For the construction of isogenic cell lines, the inducible Cas9 expression construct pCW-Cas9 (Addgene: #50661), pLKO5-sgRNA- EFS-GFP (Addgene: #57822), pLKO5-sgRNA-EFS-tRFP (Addgene: #57823), and pHIVZsgreen (Addgene: #18121) were acquired from Addgene 6,7. .. Benchling (http://benchling.com) was used to design sgRNAs targeting active domains of the human CREBBP, EP300 or KMT2D genes, as well as a neutral control region in the PPP1R12C intron 1 (at least two independent sgRNAs/gene).



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    inducible cas9 expression construct pcw cas9  (Addgene inc)
    96
    Addgene inc inducible cas9 expression construct pcw cas9
    Inducible Cas9 Expression Construct Pcw Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inducible+cas9+expression+construct+pcw+cas9/pCW-Cas9+(Plasmid+%2350661)/pmc10089214__pnas__2218330120__sapp-27-8-13
    Average 96 stars, based on 1 article reviews
    inducible cas9 expression construct pcw cas9 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

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